Cigarette smoke extract induces the proliferation of normal human urothelial cells through the NF-κB pathway.
Oncol Rep
; 35(5): 2665-72, 2016 May.
Article
en En
| MEDLINE
| ID: mdl-26883573
ABSTRACT
Bladder cancer is a common genitourinary malignant disease worldwide. Convincing evidence shows that cigarette smoke (CS) is a crucial risk factor for bladder cancer, yet the role of the NF-κB signaling pathway in the development of CS-associated bladder cancer has not been fully elucidated. In the present study, we found that exposure to cigarette smoke extract (CSE) induced proliferation and triggered the transition of normal human urothelial cells from G1 to S phase. Moreover, CSE exposure enhanced the expression of cyclin D1 and proliferating cell nuclear antigen (PCNA) and decreased the expression of p21 in SV-HUC-1 cells. Furthermore, the levels of nuclear NF-κB p65/p50 were significantly elevated by CSE. Pre-treatment with the NF-κB inhibitor (PDTC) reversed CSE-triggered cell proliferation. Taken together, our study revealed that CSE induced proliferation of normal human urothelial cells through the NF-κB pathway, and these data enhance our understanding of the CSE-related carcinogenesis of bladder cancer.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
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Fumar
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FN-kappa B
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Urotelio
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Proliferación Celular
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Oncol Rep
Año:
2016
Tipo del documento:
Article