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Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-γ, but not IL-10 Production.
Adem, Emebet; Tajebe, Fitsumbirhan; Getahun, Mulusew; Kiflie, Amare; Diro, Ermias; Hailu, Asrat; Shkedy, Ziv; Mengesha, Bewketu; Mulaw, Tadele; Atnafu, Saba; Deressa, Tekalign; Mathewos, Biniam; Abate, Ebba; Modolell, Manuel; Munder, Markus; Müller, Ingrid; Takele, Yegnasew; Kropf, Pascale.
Afiliación
  • Adem E; Department of Immunology, University of Gondar, Ethiopia.
  • Tajebe F; Department of Immunology, University of Gondar, Ethiopia.
  • Getahun M; Department of Immunology, University of Gondar, Ethiopia.
  • Kiflie A; Department of Immunology, University of Gondar, Ethiopia.
  • Diro E; Department of Internal Medicine, University of Gondar, Ethiopia.
  • Hailu A; Department of Microbiology, Immunology and Parasitology, Addis Ababa University, Ethiopia.
  • Shkedy Z; Department of Mathematics and Statistics, University of Hasselt, Belgium.
  • Mengesha B; Leishmaniasis Research and Treatment Centre, Gondar University, Ethiopia.
  • Mulaw T; Leishmaniasis Research and Treatment Centre, Gondar University, Ethiopia.
  • Atnafu S; Leishmaniasis Research and Treatment Centre, Gondar University, Ethiopia.
  • Deressa T; Department of Immunology, University of Gondar, Ethiopia.
  • Mathewos B; Department of Immunology, University of Gondar, Ethiopia.
  • Abate E; Department of Immunology, University of Gondar, Ethiopia.
  • Modolell M; Department of Cellular Immunology, Max-Planck-Institute for Immunobiology and Epigenetics, Freiburg, Germany.
  • Munder M; Third Department of Medicine (Hematology, Oncology, and Pneumology), University Medical Center Mainz, Mainz, Germany.
  • Müller I; Department of Medicine, Imperial College London, London, United Kingdom.
  • Takele Y; Leishmaniasis Research and Treatment Centre, Gondar University, Ethiopia.
  • Kropf P; Department of Medicine, Imperial College London, London, United Kingdom.
PLoS Negl Trop Dis ; 10(3): e0004468, 2016 Mar.
Article en En | MEDLINE | ID: mdl-26962865
One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-γ (IFN-γ). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-γ. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-γ and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-γ and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-γ, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-γ during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Plantas_medicinales Asunto principal: Linfocitos T / Interferón gamma / Interleucina-10 / Leishmaniasis Visceral / Antígenos de Protozoos Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies País/Región como asunto: Africa / Asia Idioma: En Revista: PLoS Negl Trop Dis Año: 2016 Tipo del documento: Article País de afiliación: Etiopia

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Plantas_medicinales Asunto principal: Linfocitos T / Interferón gamma / Interleucina-10 / Leishmaniasis Visceral / Antígenos de Protozoos Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies País/Región como asunto: Africa / Asia Idioma: En Revista: PLoS Negl Trop Dis Año: 2016 Tipo del documento: Article País de afiliación: Etiopia