Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells.
BMC Complement Altern Med
; 17(1): 24, 2017 Jan 06.
Article
en En
| MEDLINE
| ID: mdl-28056918
ABSTRACT
BACKGROUND:
Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii.METHODS:
A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV-Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis.RESULTS:
The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis.CONCLUSIONS:
These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which can serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Serratia
/
Extractos Vegetales
/
Adipocitos
/
Arándanos Azules (Planta)
/
Adipogénesis
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
BMC Complement Altern Med
Año:
2017
Tipo del documento:
Article
País de afiliación:
Canadá