Phytochemical and biological study of callus cultures of Tulbaghia violacea Harv. Cultivated in Egypt.
Nat Prod Res
; 31(15): 1717-1724, 2017 Aug.
Article
en En
| MEDLINE
| ID: mdl-28278648
ABSTRACT
As in vitro plant cultures are used extensively to produce bioactive metabolites, our goal was to establish calli from Tulbaghia violacea Harv. flowers and assess the tissue phytochemically and biologically. Murashige & Skoog medium(MS) + 22.6 µM 2,4-dichlorophenoxyacetic acid +2.2 µM benzylaminopurine induced callus from flowers. Gas chromatography/mass spectrometry(GC/MS) analyses of n-hexane extracts of calli(HC) and flowers(HF) revealed 33 and 32 components(92.6 and 98.5%, respectively). Hydrocarbons were predominant in HC (55.0%), whereas a higher percentage of oxygenated compounds was found in HF(74.6%). Trans(E)-anethole(39.1%) and 16-hentriacontanone (30.3%) dominated in HF and HC, respectively. However, sulphur compounds were only detected in HF. Quantitative estimation of thiosulphinates, phenolics, flavonoids and saponins in ethanolic extracts of calli(EC) and flowers(EF) showed much higher contents in EF. Antioxidant, antimicrobial and cytotoxic screening of extracts demonstrated that EF was the most potent, followed by HF and EC; conversely, HC was inactive. Although HC and EC were less biologically active, these calli could be an alternative source of bioactive metabolites.
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Bases de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Fitoquímicos
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Amaryllidaceae
País/Región como asunto:
Africa
Idioma:
En
Revista:
Nat Prod Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Egipto