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Effects of Bu Shen Yi sui capsule on NogoA/NgR and its signaling pathways RhoA/ROCK in mice with experimental autoimmune encephalomyelitis.
Fang, Ling; Wang, Yongqiang; Zheng, Qi; Yang, Tao; Zhao, Peiyuan; Zhao, Hui; Zhang, Qiuxia; Zhao, Yuanyuan; Qi, Fang; Li, Kangning; Chen, Zhenzhen; Li, Junling; Zhang, Nan; Fan, Yongping; Wang, Lei.
Afiliación
  • Fang L; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Wang Y; Daxing Hospital Affiliated to Capital Medical University, Beijing, 102600, People's Republic of China.
  • Zheng Q; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Yang T; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Zhao P; Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing, 100053, People's Republic of China.
  • Zhao H; Beijing Tian Tan Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.
  • Zhang Q; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Zhao Y; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Qi F; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Li K; Core Facility Center, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Chen Z; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Li J; Beijing Tian Tan Hospital, Capital Medical University, Beijing, 100050, People's Republic of China.
  • Zhang N; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Fan Y; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
  • Wang L; School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing, 100069, People's Republic of China.
BMC Complement Altern Med ; 17(1): 346, 2017 Jul 01.
Article en En | MEDLINE | ID: mdl-28668079
BACKGROUND: Axon growth inhibitory factors NogoA/Nogo receptor (NgR) and its signaling pathways RhoA/Rho kinase (ROCK) play a critical role in the repair of nerve damage in multiple sclerosis (MS). Bu Shen Yi Sui Capsule (BSYSC) is an effective Chinese formula utilized to treat MS in clinical setting and noted for its potent neuroprotective effects. In this study, we focus on the effects of BSYSC on promoting nerve repair and the underlying mechanisms in mice with experimental autoimmune encephalomyelitis (EAE), an animal model of MS. METHODS: The EAE mouse model was induced by injecting subcutaneously with myelin oligodendrocyte glycoprotein (MOG) 35-55 supplemented with pertussis toxin. BSYSC was orally administrated at dose of 3.0 g/kg once a day for 40 days. The levels of protein gene product (PGP) 9.5, p-Tau, growth associated protein (GAP) -43, KI67 and Nestin in the brain or spinal cord on 20 and 40 day post-induction (dpi) were detected via immunofluorescence and Western blot analysis. Furthermore, NogoA/NgR and RhoA/ROCK signaling molecules were studied by qRT-PCR and Western blot analysis. RESULTS: Twenty or 40 days of treatment with BSYSC increased markedly PGP9.5 and GAP-43 levels, reduced p-Tau in the brain or spinal cord of mice with EAE. In addition, BSYSC elevated significantly the expression of KI67 and Nestin in the spinal cord 40 dpi. Further study showed that the activation of NogoA/NgR and RhoA/ROCK were suppressed by the presence of BSYSC. CONCLUSIONS: BSYSC could attenuate axonal injury and promote repair of axonal damage in EAE mice in part through the down-regulation of NogoA/NgR and RhoA/ROCK signaling pathways.
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Texto completo: 1 Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Asunto principal: Medicamentos Herbarios Chinos / Proteína de Unión al GTP rhoA / Encefalomielitis Autoinmune Experimental / Quinasas Asociadas a rho / Receptores Nogo / Proteínas Nogo Tipo de estudio: Prognostic_studies Idioma: En Revista: BMC Complement Altern Med Año: 2017 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Asunto principal: Medicamentos Herbarios Chinos / Proteína de Unión al GTP rhoA / Encefalomielitis Autoinmune Experimental / Quinasas Asociadas a rho / Receptores Nogo / Proteínas Nogo Tipo de estudio: Prognostic_studies Idioma: En Revista: BMC Complement Altern Med Año: 2017 Tipo del documento: Article