Structural characterization and osteogenic bioactivity of a sulfated polysaccharide from pacific abalone (Haliotis discus hannai Ino).
Carbohydr Polym
; 182: 207-214, 2018 Feb 15.
Article
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| MEDLINE
| ID: mdl-29279116
ABSTRACT
Bone morphogenic protein-2 (BMP-2) is known to promote osteogenesis. To find novel adjuvants to enhance the activity of BMP-2, the present study investigated the structure BMP-2-induced osteogenic activity of a water-soluble polysaccharide from the gonad of pacific abalone (Haliotis discus hannai Ino) named AGSP. Through analysis of aldobiouronic acids released from AGSP, monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as â3)-GlcA(1â3)-Gal(1â with sulfated branches comprised of prevelant Gal and minor Glc, and â4)-ß-GlcA(1â2)-α-Man(1â residue was also found. AGSP possessed a sulfate content of 12.4% with a relative molecular weight of 6.6kDa. AGSP strengthened alkaline phosphatase activity induced by BMP-2 in a dose dependent manner at 10-200µg/mL with 425% enhancement being observed at 200µg/mL, indicating AGSP could be an adjuvant candidate to enhance osteogenic activity of BMP-2.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Polisacáridos
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Sulfatos
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Gastrópodos
Idioma:
En
Revista:
Carbohydr Polym
Año:
2018
Tipo del documento:
Article