Serum containing Gengnianchun formula suppresses amyloid ßinduced inflammatory cytokines in BV2 microglial cells by inhibiting the NFκB and JNK signaling pathways.
Mol Med Rep
; 17(4): 5043-5048, 2018 04.
Article
en En
| MEDLINE
| ID: mdl-29393440
ABSTRACT
As the resident macrophages of the brain's innate immune system, microglial cells are key modulators in the neurodegenerative disease Alzheimer's disease (AD). In particular, the activation and accumulation of microglial cells around amyloid plaques is considered to result in chronic neuroinflammation. Although the pathologic mechanism remains to be fully elucidated, inflammation has been shown to be critical in the pathogenesis of AD. The Gengnianchun (GNC) formula has long been used to treat perimenopausal syndrome clinically, and is particularly effective in improving learning ability and memory. Our previous study demonstrated that GNC formula had an antiinflammatory effect and offered neuroprotection in animal experiments. In the present study, the antiinflammatory properties of GNC and its underlying mechanism of action were examined in BV2 microglial cells. Amyloidß peptide (Aß)stimulated microglial cells were examined for the production of proinflammatory cytokines and the underlying signaling pathways. Compared with the normal control group, the protein expression levels of IL1ß and TNFα were significantly increased following treatment with Aß (P<0.01), but medicated rat serum containing GNC formula (MRS) could significantly attenuated the Aßinduced secretion of these proinflammatory cytokines. It was identified by CCK8 assay that the viability of the BV2 cells was not reduced following treatment with various concentrations of MRS. The phosphorylation of factorκB (NFκB) and cJun Nterminal kinase (JNK) was markedly increased following treatment with Aß, compared with the normal control group (P<0.01). However, treatment with MRS resulted in a significant reduction in the phosphorylation of NFκB (P<0.05). These results suggested that MRS suppressed the Aßinduced inflammatory response of microglial cells by inhibiting the NFκB and JNK signaling pathways. These novel findings provide insights into the development of GNC formula as a therapeutic agent for the treatment of neurodegenerative disorders.
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Texto completo:
1
Bases de datos:
MEDLINE
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
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Medicina_china
Asunto principal:
Medicamentos Herbarios Chinos
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Transducción de Señal
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Péptidos beta-Amiloides
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Citocinas
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FN-kappa B
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Microglía
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Mediadores de Inflamación
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Proteínas Quinasas JNK Activadas por Mitógenos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article