Antimycobacterial potential of the juniper berry essential oil in tap water.
Arh Hig Rada Toksikol
; 69(1): 46-54, 2018 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-29604199
ABSTRACT
Mycobacterium avium complex-related diseases are often associated with poorly maintained hot water systems. This calls for the development of new control strategies. The aim of this study was to investigate the activity of essential oils (EOs) from the Mediterranean plants, common juniper, immortelle, sage, lavandin, laurel, and white cedar against Mycobacterium avium ssp. avium, Mycobacterium intracellulare, and Mycobacterium gordonae in culturing broth and freshwater as their most common habitat. To do that, we developed a new method of water microdilution to determine their minimal effective concentrations (MEC). The most active EO was the one from the common juniper with the MEC of 1.6 mg mL-1. Gas chromatography / mass spectrometry the juniper EO identified monoterpenes (70.54 %) and sesquiterpenes (25.9 %) as dominant component groups. The main monoterpene hydrocarbons were α-pinene, sabinene, and ß-pinene. The juniper EO significantly reduced the cell viability of M. intracellulare and M. gordonae at MEC, and of M. avium at 2xMEC. Microscopic analysis confirmed its inhibitory effect by revealing significant morphological changes in the cell membrane and cytoplasm of all three bacteria. The mode of action of the juniper EO on the cell membrane was confirmed by a marked leakage of intracellular material. Juniper EO has a great practical potential as a complementary or alternative water disinfectant in hot water systems such as baths, swimming pools, spa pools, hot tubs, or even foot baths/whirlpools.
Palabras clave
Helichrysum italicum; Juniperus communis; Laurus nobilis; Lavandula hybrida; Mycobacterium avium; Mycobacterium gordonae; Mycobacterium intracellulare; Salvia officinalis; Thuja occidentalis; minimum effective concentration; nontuberculous mycobacteria; tap water; water infections; water microdilution
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microbiología del Agua
/
Agua Potable
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Aceites de Plantas
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Aceites Volátiles
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Juniperus
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Frutas
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Antibacterianos
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Mycobacterium
País/Región como asunto:
Europa
Idioma:
En
Revista:
Arh Hig Rada Toksikol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Croacia