Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract.
Biosci Biotechnol Biochem
; 82(7): 1172-1175, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29658432
Palabras clave
Acidic PNGase; FNG: free N-glycan; Fuc: L-fucose; Gal: D-galactose; GlcNAc: N-acetyl-D-glucosamine; HPLC: high-performance liquid chromatography; Man: D-mannose; NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAc; NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAcß14GlcNAc; NeuNAc: N-acetylneuraminic acid; PA-: pyridylamino; PNGase-A: aPNGase from almond seed; PNGase-Le: aPNGase from tomato (Solanum lycopersium L.); PNGase: peptide:N-glycanase; PTC: plant complex type; RCA120: Ricinus communis agglutinin (120 kDa); RP-HPLC: reversed-phase HPLC; SF-HPLC: size-fractionation HPLC; Xyl: D-xylose; affinity chromatography; enzyme assay; free N-glycans; transgenic plant
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1
Bases de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón