Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract.

Uemura, Ryota; Ogura, Mikako; Matsumaru, Chihiro; Akiyama, Tsuyoshi; Maeda, Megumi; Kimura, Yoshinobu

Uemura, Ryota; Ogura, Mikako; Matsumaru, Chihiro; Akiyama, Tsuyoshi; Maeda, Megumi; Kimura, Yoshinobu.

Afiliación

Uemura R; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Ogura M; b Faculty of Agriculture , Okayama University , Okayama , Japan.
Matsumaru C; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Akiyama T; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Maeda M; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Kimura Y; b Faculty of Agriculture , Okayama University , Okayama , Japan.

Biosci Biotechnol Biochem ; 82(7): 1172-1175, 2018 Jul.

Article en En | MEDLINE | ID: mdl-29658432

Asunto(s)

Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa/metabolismo; Extractos Vegetales/química; Secuencia de Aminoácidos; Arabidopsis/química; Cromatografía Liquida/métodos; Colorantes Fluorescentes/química; Glicopéptidos/química; Glicopéptidos/metabolismo; Solanum lycopersicum/química; Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa/genética; Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa/aislamiento & purificación; Hojas de la Planta/química; Plantas Modificadas Genéticamente; Especificidad por Sustrato

Palabras clave

Acidic PNGase; FNG: free N-glycan; Fuc: L-fucose; Gal: D-galactose; GlcNAc: N-acetyl-D-glucosamine; HPLC: high-performance liquid chromatography; Man: D-mannose; NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAc; NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAcß14GlcNAc; NeuNAc: N-acetylneuraminic acid; PA-: pyridylamino; PNGase-A: aPNGase from almond seed; PNGase-Le: aPNGase from tomato (Solanum lycopersium L.); PNGase: peptide:N-glycanase; PTC: plant complex type; RCA120: Ricinus communis agglutinin (120 kDa); RP-HPLC: reversed-phase HPLC; SF-HPLC: size-fractionation HPLC; Xyl: D-xylose; affinity chromatography; enzyme assay; free N-glycans; transgenic plant

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Extractos Vegetales / Péptido-N4-(N-acetil-beta-glucosaminil) Asparagina Amidasa Idioma: En Revista: Biosci Biotechnol Biochem Año: 2018 Tipo del documento: Article País de afiliación: Japón

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