Ginkgo biloba sarcotesta polysaccharide inhibits inflammatory responses through suppressing both NF-κB and MAPK signaling pathway.
J Sci Food Agric
; 99(5): 2329-2339, 2019 Mar 30.
Article
en En
| MEDLINE
| ID: mdl-30338529
ABSTRACT
BACKGROUND:
Polysaccharides, common components of natural products extensively studied as dietary supplements and functional foods, have been found to have various activities. In the present study, a water-soluble polysaccharide, namely GBSP3a, was isolated and purified from G. biloba sarcotesta. The anti-inflammatory activity of GBSP3a in lipopolysaccharide (LPS)-induced RAW264.7 macrophages and the potential underlying molecular mechanisms were then assessed.RESULTS:
GBSP3a exerted its anti-inflammatory effect by remarkably inhibiting the secretion of pro-inflammatory mediators and cytokines, including nitric oxide (NO), prostaglandin E2 (PGE2 ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1ß (IL-1ß) in LPS-stimulated RAW264.7 macrophages. Excessive mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dose-dependently inhibited by GBSP3a in LPS-stimulated RAW264.7 cells. Further research suggested that the anti-inflammatory effect of GBSP3a can be attributed to the modulation of the NF-κB and MAPK signaling pathways.CONCLUSION:
GBSP3a exhibits anti-inflammatory activity and exerts its anti-inflammatory effect probably through suppressing both NF-κB and MAPK signaling pathway, indicating that GBSP3a could be used for the development of anti-inflammatory agent or nutraceuticals. © 2018 Society of Chemical Industry.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polisacáridos
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Extractos Vegetales
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FN-kappa B
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Ginkgo biloba
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Macrófagos
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Antiinflamatorios
Idioma:
En
Revista:
J Sci Food Agric
Año:
2019
Tipo del documento:
Article
País de afiliación:
China