Electroacupuncture Regulates Hippocampal Synaptic Plasticity via Inhibiting Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling in Cerebral Ischemic Rats.
J Stroke Cerebrovasc Dis
; 28(3): 792-799, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30552029
ABSTRACT
OBJECTIVE:
To determine the mechanism(s) involved in electroacupuncture (EA)-mediated improvements in synaptic plasticity in a rat model of middle cerebral artery occlusion and reperfusion (MCAO/R)-induced cognitive deficits.METHODS:
Focal cerebral ischemic stroke was induced by (MCAO/R) surgery. Rats were randomly split into 4 groups control group (sham operation control), MCAO group, Baihui (GV 20) and Shenting (GV 24) acupoint EA group (verum acupuncture, MCAOâ¯+â¯VA), and nonacupoint EA group (control acupuncture, MCAOâ¯+â¯CA). EA treatment was administered for 14 consecutive days in MCAOâ¯+â¯VA and MCAOâ¯+â¯CA groups. Neurological assessment, behavioral performance testing, and molecular biology assays were used to evaluate the MCAO/R model, EA therapeutic effect and potential therapeutic mechanism(s) of EA.RESULTS:
Significant amelioration of neurological deficits was found in MCAOâ¯+â¯VA rats compared with MCAO rats (P < .01). Moreover, learning and memory significantly improved in EA-treated rats compared with MCAO or MCAOâ¯+â¯CA rats (P < .05) together with an increase in the number of PSD-95+ and SYN+ cells and synapses in the hippocampal CA1 region (P < .05). MCAOâ¯+â¯VA rats also showed amelioration of pathological synaptic ultrastructural changes compared with MCAO or MCAOâ¯+â¯CA groups (P < .001). In contrast, EA decreased the levels and phosphorylation of JAK2 (Janus-activated kinase 2) and STAT3 (signal transducer and activator of transcription 3) in the hippocampal CA1 region compared with MCAO or MCAOâ¯+â¯CA group (P < .01).CONCLUSION:
EA at GV 20 and GV 24 acupoints improved cognitive deficits in cerebral ischemic rats via the JAK2/STAT3 signaling pathway and mediated synaptic plasticity in the peri-infarct hippocampal CA1 region of rats following ischemic stroke.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapéuticos y Terapias MTCI:
Terapias_energeticas
Asunto principal:
Electroacupuntura
/
Infarto de la Arteria Cerebral Media
/
Factor de Transcripción STAT3
/
Janus Quinasa 2
/
Región CA1 Hipocampal
/
Plasticidad Neuronal
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Stroke Cerebrovasc Dis
Año:
2019
Tipo del documento:
Article