LC-MS/MS determination of ginsenoside compound K and its metabolite 20 (S)-protopanaxadiol in human plasma and urine: applications in a clinical study.
Bioanalysis
; 11(5): 365-380, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30873858
ABSTRACT
AIM:
Ginsenoside compound K (CK) is considered to be a potential therapeutic drug for rheumatoid arthritis because of its good anti-inflammatory activity. The purpose of this work was to establish a rapid, sensitive and specific method for determination of CK and its active metabolite 20(S)-protopanaxadiol (20(S)-PPD). Materials &methods:
The analytes and internal standards were extracted by liquid-liquid extraction. Then, were separated by high performance liquid phase and determined by triple quadrupole mass spectrometry.RESULTS:
A LC-MS/MS using liquid-liquid extraction was developed for determining CK over the concentration range 1.00-1002.00 ng/ml and 0.15-54.30 ng/ml for 20(S)-PPD. The lower limits of quantification for CK and 20(S)-PPD were 1.00 and 0.15 ng/ml, respectively.CONCLUSION:
This method was successfully validated for detecting both CK and 20(S)-PPD in the human plasma and urine, and was proved to be suitable for the pharmacokinetic study of CK in healthy Chinese volunteers. CLINICAL TRIAL REGISTRATION NUMBER ChiCTR-TRC-14004824.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
/
Plantas_medicinales
Asunto principal:
Sapogeninas
/
Cromatografía Liquida
/
Ginsenósidos
/
Espectrometría de Masas en Tándem
/
Panax
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Bioanalysis
Año:
2019
Tipo del documento:
Article