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Velutin, an Aglycone Extracted from Korean Mistletoe, with Improved Inhibitory Activity against Melanin Biosynthesis.
Jung, Se-Hui; Kim, Jaehyun; Eum, Juneyong; Choe, Jung Won; Kim, Hak Hyun; Kee, Yun; Lee, Kooyeon.
Afiliación
  • Jung SH; Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
  • Kim J; Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
  • Eum J; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
  • Choe JW; Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
  • Kim HH; Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea.
  • Kee Y; Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea. yunkee@kangwon.ac.kr.
  • Lee K; Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea. lky@kangwon.ac.kr.
Molecules ; 24(14)2019 Jul 12.
Article en En | MEDLINE | ID: mdl-31336931
The abnormal regulation of melanin synthesis leads to a wide range of pigmentary disorders. Although various melanin biosynthesis inhibitors have been developed, their efficacy and long-term safety needs to be further improved, and thus the goal of this study is to develop promising natural compound inhibitors of melanin biosynthesis. Here, we obtained aglycone flavonoid extract through the microwave-assisted hydrolysis of glycoside extract from Korean mistletoe in acidic condition. The aglycone extract inhibited tyrosinase activity more efficiently with better antioxidant activity than glycoside extract in vitro. The microwave-assisted aglycone extract of mistletoe was further analyzed for in vivo activity, and the results showed the aglycone extract inhibited both early melanocyte development and melanin synthesis more efficiently in zebrafish embryo in a dose-dependent manner. Our in vivo toxicity assay quantitatively measured cell death in zebrafish embryos and showed that the microwave-assisted aglycone extract of mistletoe had no significant effect on cell death (p < 0.001), indicating that aglycone extract is more biocompatible than glycoside extract. Furthermore, our in vitro and in vivo analyses successfully identified and characterized velutin, an aglycone of a homoflavoyadorinin B glycoside, as a major inhibitory component in the microwave-assisted mistletoe extract. Ultimately, this study showed that the novel natural compound inhibitor velutin, which was generated through microwave-assisted extraction from mistletoe, improved the efficacy of melanin biosynthesis inhibition with little toxicity.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Muérdago / Extractos Vegetales / Flavonas / Vías Biosintéticas / Melaninas Idioma: En Revista: Molecules Año: 2019 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Muérdago / Extractos Vegetales / Flavonas / Vías Biosintéticas / Melaninas Idioma: En Revista: Molecules Año: 2019 Tipo del documento: Article