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Evaluating the efficacy of an algae-based treatment to mitigate elicitation of antibiotic resistance.
Grimes, Kassandra L; Dunphy, Laura J; Loudermilk, Erica M; Melara, A Jasmin; Kolling, Glynis L; Papin, Jason A; Colosi, Lisa M.
Afiliación
  • Grimes KL; Department of Civil and Environmental Engineering, University of Virginia, P.O. Box 400742, Charlottesville, VA, 22904-4742, United States.
  • Dunphy LJ; Department of Biomedical Engineering, University of Virginia, P.O. Box 800759, Charlottesville, VA, 22908-0759, United States.
  • Loudermilk EM; Department of Civil and Environmental Engineering, University of Virginia, P.O. Box 400742, Charlottesville, VA, 22904-4742, United States.
  • Melara AJ; Department of Civil and Environmental Engineering, University of Virginia, P.O. Box 400742, Charlottesville, VA, 22904-4742, United States.
  • Kolling GL; Department of Biomedical Engineering, University of Virginia, P.O. Box 800759, Charlottesville, VA, 22908-0759, United States; Department of Medicine, Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, 22903, United States.
  • Papin JA; Department of Biomedical Engineering, University of Virginia, P.O. Box 800759, Charlottesville, VA, 22908-0759, United States.
  • Colosi LM; Department of Civil and Environmental Engineering, University of Virginia, P.O. Box 400742, Charlottesville, VA, 22904-4742, United States. Electronic address: lmc6b@virginia.edu.
Chemosphere ; 237: 124421, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31382196
Antibiotics in the effluents of municipal wastewater treatment plants (WWTP) may create selective pressures to induce antibiotic resistance in bacteria downstream. This study evaluates ciprofloxacin (CIP) removal by a freshwater alga, Scenedesmus dimorphus, to assess the efficacy of algae-based tertiary treatment in reducing effluent-induced CIP resistance. Results show significant CIP removal in light-exposed samples without algae and experimental algae (EA) samples: 53% and 93%, respectively, over 144 h. A residual antibiotic potency assay reveals that untreated CIP is significantly more growth-inhibiting to a model bacterium (Escherichia coli) than the algae-treated and light-exposed samples during short exposures (6 h). Adaptive laboratory evolution (ALE), again using E. coli, reveals that treated samples exhibit reduced capacity to elicit CIP resistance during sustained exposures compared to untreated CIP. Finally, observed CIP resistance in the CIP-exposed ALE lineages is corroborated via genotype characterization, which reveals the presence of resistance-associated mutations in gyrase subunit A (gyrA) that are not present in ALE lineages exposed to algae treated or light-exposed samples. As such, algae-mediated tertiary treatment could be effective in suppressing CIP resistance in bacterial communities downstream from WWTP. In addition, ALE is useful for assessing the potential of wastewater-relevant samples to elicit antibiotic resistance downstream.
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Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas / Aromoterapia / Plantas_medicinales Asunto principal: Ciprofloxacina / Eliminación de Residuos Líquidos / Farmacorresistencia Bacteriana / Scenedesmus / Antibacterianos Idioma: En Revista: Chemosphere Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas / Aromoterapia / Plantas_medicinales Asunto principal: Ciprofloxacina / Eliminación de Residuos Líquidos / Farmacorresistencia Bacteriana / Scenedesmus / Antibacterianos Idioma: En Revista: Chemosphere Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos