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Multiple In vitro biological effects of phenolic compounds from Morus alba root bark.
Culenová, Marie; Sychrová, Alice; Hassan, Sherif T S; Berchová-Bímová, Katerina; Svobodová, Petra; Helclová, Alexandra; Michnová, Hana; Hosek, Jan; Vasilev, Hristo; Suchý, Pavel; Kuzminová, Gabriela; Svajdlenka, Emil; Gajdziok, Jan; Cízek, Alois; Suchý, Václav; Smejkal, Karel.
Afiliación
  • Culenová M; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic. Electronic address: culenovam@vfu.cz.
  • Sychrová A; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Hassan STS; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Berchová-Bímová K; Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, CZ-165 21, Praha 6-Suchdol, Czech Republic.
  • Svobodová P; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Helclová A; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Michnová H; Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Hosek J; Department of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Vasilev H; Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav str., BG-1000, Sofia, Bulgaria.
  • Suchý P; Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Kuzminová G; Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Svajdlenka E; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Gajdziok J; Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Cízek A; Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Suchý V; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic.
  • Smejkal K; Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, CZ-612 42, Brno, Czech Republic. Electronic address: karel.mejkal@post.cz.
J Ethnopharmacol ; 248: 112296, 2020 Feb 10.
Article en En | MEDLINE | ID: mdl-31610262
ETHNOPHARMACOLOGICAL RELEVANCE: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. AIM OF THE STUDY: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. MATERIALS AND METHODS: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. RESULTS: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 µg/mL, and EC50 values 0.93 and 1.61 µg/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 µg/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. CONCLUSIONS: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.
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Texto completo: 1 Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Extractos Vegetales / Morus / Diabetes Mellitus / Hipoglucemiantes Idioma: En Revista: J Ethnopharmacol Año: 2020 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Extractos Vegetales / Morus / Diabetes Mellitus / Hipoglucemiantes Idioma: En Revista: J Ethnopharmacol Año: 2020 Tipo del documento: Article