Comparison of the use of d-enantiomeric and l-enantiomeric antimicrobial peptides incorporated in a calcium-chelating irrigant against Enterococcus faecalis root canal wall biofilms.
J Dent
; 91: 103231, 2019 12.
Article
en En
| MEDLINE
| ID: mdl-31712128
ABSTRACT
OBJECTIVES:
To compare the anti-biofilm efficacy of two antimicrobial peptides (AMPs), 1018 and DJK-5, in disrupting canal wall biofilms in the isthmus, canal and dentinal tubules of single-rooted maxillary premolars.METHODS:
Enterococcus faecalis single-species biofilms were formed in-situ in the root canal system of the premolars (nâ¯=â¯91). Confocal laser scanning microscopy, bacterial sampling, colony-forming unit counting, XTT assay, lactate dehydrogenase assay and phenol-sulphuric acid method were used to identify the anti-biofilm efficacy of both AMPs and their influence on bacterial metabolic activity.RESULTS:
Both AMPs disrupted in-situ E. faecalis biofilms and altered their metabolic activity. At 20⯵g/mL, the d-enantiomeric AMP DJK-5 killed 55.5 %, 57.3 % and 55.8 % of biofilm bacteria in the isthmus, canal and dentinal tubules, respectively, in 1â¯min. In contrast, the l-enantiomeric AMP 1018 only eradicated 25.6 %, 25.5 % and 27.5 % of biofilm bacteria in the isthmus, canal and dentinal tubules, respectively, within the same time. Anti-biofilm efficacy of the root canal irrigants tested were in the order 6 % NaOCl > 20⯵g/mL DJK-5â¯>â¯10⯵g/mL DJK-5â¯>â¯20⯵g/mL 1018â¯>â¯10⯵g/mL 1018â¯>â¯0.9 % NaCl.CONCLUSIONS:
The present results are confirmatory of previous studies, in that d-enantiomeric AMPs exhibit more potent antibacterial properties than l-enantiomeric AMPs against E. faecalis biofilms within the canal space. Nevertheless, the potency of both AMPs are concentration-dependent. Incorporation of these agents into EDTA, a non-antibacterial calcium-chelating irrigant for removal of the inorganic component of the canal space debris, does not reduce the efficacy of either AMP. CLINICALSIGNIFICANCE:
The present study provides the proof of concept that incorporation of an antimicrobial peptide into a calcium-chelating root canal irrigant enhances the disinfection of intratubular single-species biofilms during smear layer and smear plug removal.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Métodos Terapéuticos y Terapias MTCI:
Plantas_medicinales
Asunto principal:
Péptidos
/
Irrigantes del Conducto Radicular
/
Enterococcus faecalis
/
Biopelículas
/
Cavidad Pulpar
/
Antibacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Dent
Año:
2019
Tipo del documento:
Article
País de afiliación:
China