Phase Diagram for a Lysyl-Phosphatidylglycerol Analogue in Biomimetic Mixed Monolayers with Phosphatidylglycerol: Insights into the Tunable Properties of Bacterial Membranes.
Chemphyschem
; 21(8): 702-706, 2020 04 20.
Article
en En
| MEDLINE
| ID: mdl-32065707
ABSTRACT
Ion pairing between the major phospholipids of the Staphylococcus aureus plasma membrane (phosphatidylglycerol - PG and lysyl-phosphatidylglycerol - LPG) confers resistance to antimicrobial peptides and other antibiotics. We developed 3adLPG, a stable synthetic analogue which can substitute for the highy-labile native LPG, in biophysical experiments examining the membrane-protecting role of lipid ion pairing, in S. aureus and other important bacteria. Here we examine the surface charge and lipid packing characteristics of synthetic biomimetic mixtures of DPPG and DP3adLPG in Langmuir monolayers, using a combination of complementary surface-probing techniques such as infrared reflection-absorption spectroscopy and grazing-incidence x-ray diffraction. The resultant phase diagram for the ion paired lipids sheds light on the mixing behavior of lipids in monolayer models of resistant phenotype bacterial membranes, and provides a platform for future biophysical studies.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfatidilgliceroles
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Staphylococcus aureus
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Materiales Biomiméticos
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Membrana Dobles de Lípidos
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Lisina
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Lípidos de la Membrana
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Membranas Artificiales
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Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Chemphyschem
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania