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Pomegranate Seeds Extract Possesses a Protective Effect against Tramadol-Induced Testicular Toxicity in Experimental Rats.
Minisy, Fatma M; Shawki, Hossam H; El Omri, Abdelfatteh; Massoud, Ahmed A; Omara, Enayat A; Metwally, Fatma G; Badawy, Manal A; Hassan, Neveen A; Hassan, Nabila S; Oishi, Hisashi.
Afiliación
  • Minisy FM; Department of Comparative and Experimental Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
  • Shawki HH; Department of Pathology, Medical Division, National Research Center, Cairo, Egypt.
  • El Omri A; Department of Comparative and Experimental Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
  • Massoud AA; National Gene Bank of Egypt (NGB), Agricultural Research Center (ARC), Giza, Egypt.
  • Omara EA; Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia.
  • Metwally FG; Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
  • Badawy MA; Department of Pathology, Medical Division, National Research Center, Cairo, Egypt.
  • Hassan NA; Department of Academic Science, Research Institute of Ophthalmology, Giza, Egypt.
  • Hassan NS; Department of Pathology, Medical Division, National Research Center, Cairo, Egypt.
  • Oishi H; National Gene Bank of Egypt (NGB), Agricultural Research Center (ARC), Giza, Egypt.
Biomed Res Int ; 2020: 2732958, 2020.
Article en En | MEDLINE | ID: mdl-32219129
Tramadol is a centrally acting opioid analgesic that is extensively used. The chronic exposure to tramadol induces oxidative stress and toxicity especially for patients consuming it several times a day. Previously, we and others reported that tramadol induces testicular damage in rats. This study was conducted to investigate the possible protective effect of pomegranate seed extract (PgSE) against tramadol-induced testicular damage in adult and adolescent rats. Male rats were orally treated with tramadol or in a combination with PgSE for three weeks. Testes were then dissected and analyzed. Histological and ultrastructural examinations indicated that tramadol induced many structural changes in the testes of adult and adolescent rats including hemorrhage of blood vessels, intercellular spaces, interstitial vacuoles, exfoliation of germ cells in lumen, cell apoptosis, chromatin degeneration of elongated spermatids, and malformation of sperm axonemes. Interestingly, these abnormalities were not observed in tramadol/PgSE cotreated rats. The morphometric analysis revealed that tramadol disrupted collagen metabolism by elevating testicular levels of collagen fibers but that was protected in tramadol/PgSE cotreatment at both ages. In addition, DNA ploidy revealed that S phase of the cell cycle was diminished when adult and adolescent rats were treated with tramadol. However, the S phase had a normal cell population in the cotreated adult rats, but adolescent rats had a lower population than controls. Furthermore, the phytochemistry of PgSE revealed a high content of total polyphenols and total flavonoids within this extract; besides, the DPPH free radical scavenging activity was high. In conclusion, this study indicated that PgSE has a prophylactic effect against tramadol-induced testicular damage in both adult and adolescent ages, although the tramadol toxicity was higher in adolescent age to be completely protected. This prophylactic effect might be due to the high antioxidant compounds within the pomegranate seeds.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Semillas / Enfermedades Testiculares / Testículo / Tramadol / Extractos Vegetales / Granada (Fruta) Idioma: En Revista: Biomed Res Int Año: 2020 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Semillas / Enfermedades Testiculares / Testículo / Tramadol / Extractos Vegetales / Granada (Fruta) Idioma: En Revista: Biomed Res Int Año: 2020 Tipo del documento: Article País de afiliación: Japón