Bifidobacterium breve UCC2003 Exopolysaccharide Modulates the Early Life Microbiota by Acting as a Potential Dietary Substrate.
Nutrients
; 12(4)2020 Mar 29.
Article
en En
| MEDLINE
| ID: mdl-32235410
ABSTRACT
BACKGROUND:
Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe-microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system.METHODS:
Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR).RESULTS:
Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel.CONCLUSIONS:
These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
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Colon
/
Suplementos Dietéticos
/
Microbioma Gastrointestinal
/
Salud del Lactante
/
Bifidobacterium breve
/
Interacciones Microbiota-Huesped
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nutrients
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido