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The effect of hyperbaric oxygen therapy on bone macroscopy, composition and biomechanical properties after ionizing radiation injury.
Júnior, Luiz Henrique Ferreira; Limirio, Pedro Henrique Justino Oliveira; Soares, Priscilla Barbosa Ferreira; Dechichi, Paula; de Souza Castro Filice, Letícia; Quagliatto, Paulo Sérgio; Rocha, Flaviana Soares.
Afiliación
  • Júnior LHF; Integrated Dental Clinic Program, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • Limirio PHJO; Integrated Dental Clinic Program, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • Soares PBF; Department of Periodontology and Oral Implantology, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/nº, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • Dechichi P; Department of Cell Biology, Histology and Embryology, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/nº, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • de Souza Castro Filice L; Department of Clinical Medicine, Histology and Embryology, Faculty of Medicine, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4U, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • Quagliatto PS; Department of Dentistry and Dental Materials, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/nº, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil.
  • Rocha FS; Department of Oral and Maxillofacial Surgery and Traumatology and Implantology, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará s/nº, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil. flavianasoares.rocha@gmail.com.
Radiat Oncol ; 15(1): 95, 2020 May 06.
Article en En | MEDLINE | ID: mdl-32375798
BACKGROUND: Radiotherapy used in tumor treatment compromises vascularization of bone tissue. Hyperbaric oxygenation (HBO) increases oxygen availability and improves vascularization, minimizing the deleterious effects of ionizing radiation (IR). Therefore, the aim of this study was to evaluate HBO therapy effect on bone macroscopy, composition and biomechanical properties after IR damage. METHODS: Twenty male Wistar rats weighing 300 ± 20 g (10 weeks of age) were submitted to IR (30 Gy) to the left leg, where the right leg was not irradiated. After 30 days, ten animals were submitted to HBO therapy, which was performed daily for 1 week at 250 kPa for 90-min sessions. All animals were euthanized 37 days after irradiation and the tibia were separated into four groups (n = 10): from animals without HBO - right tibia Non-irradiated (noIRnoHBO) and left tibia Irradiated (IRnoHBO); and from animals with HBO - right tibiae Non-irradiated (noIRHBO) and left tibia Irradiated (IRHBO). The length (proximal-distal) and thickness (anteroposterior and mediolateral) of the tibiae were measured. Biomechanical analysis evaluated flexural strength and stiffness. Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) was used to calculate the amide I ratio, crystallinity index, and matrix to mineral ratios. RESULTS: In the macroscopic and ATR-FTIR analysis, the IRnoHBO showed lower values of length, thickness and amide I ratio, crystallinity index and matrix to mineral ratios compared to noIRnoHBO (p < 0.03). IRnoHBO showed no statistical difference compared to IRHBO for these analyses (p > 0.05). Biomechanics analysis showed that the IRnoHBO group had lower values of flexural strength and stiffness compared to noIRnoHBO and IRHBO groups (p < 0.04). In addition, the noIRHBO group showed higher value of flexural strength when compared to noIRnoHBO and IRHBO groups (p < 0.02). CONCLUSIONS: The present study concluded that IR arrests bone development, decreases the collagen maturation and mineral deposition process, thus reducing the flexural strength and stiffness bone mechanical parameters. Moreover, HBO therapy minimizes deleterious effects of irradiation on flexural strength and the bone stiffness analysis.
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Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas / Oxigenoterapia_hiperbrica Asunto principal: Traumatismos por Radiación / Huesos / Oxigenoterapia Hiperbárica Idioma: En Revista: Radiat Oncol Año: 2020 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas / Oxigenoterapia_hiperbrica Asunto principal: Traumatismos por Radiación / Huesos / Oxigenoterapia Hiperbárica Idioma: En Revista: Radiat Oncol Año: 2020 Tipo del documento: Article País de afiliación: Brasil