Calcium-cation-doped polydopamine-modified 2D black phosphorus nanosheets as a robust platform for sensitive and specific biomolecule sensing.
Anal Chim Acta
; 1121: 1-10, 2020 Jul 18.
Article
en En
| MEDLINE
| ID: mdl-32493583
ABSTRACT
Many polymer decorated/modified 2D nanomaterials have been developed as enhanced drug delivery systems and photothermal theranostic nanoagents. However, few reports describe the use of these novel nanomaterials as nanoplatforms for biomolecule sensing. Herein, we used calcium-cation-doped polydopamine-modified (PDA-modified) 2D black phosphorus (BP) nanosheets (BP@PDA) as a sensing nanoplatform for the detection of nucleic acids and proteins in complex biological samples. Fluorescent-dye-labeled single-strand DNA aptamer/probes are adsorbed by the Ca2+-doped BP@PDA mediated by calcium-cation coordination. The PDA coating enhances the stability of the inner BP, provides binding sites to DNA nucleobases, and quenches fluorescence. Without any chemical conjugation, this sensing nanoplatform selectively and specifically detects protein (human thrombin, linear range 10-25 nM, detection limit 0.02 nM), single-strand DNA (linear range 1-10 nM, detection limit 0.52 nM) in 1% serum diluted samples, and senses intracellular mRNAs (C-myc, and actin) in living cells. The nanoplatform exhibits the advantages of both the 2D nanomaterial (BP) and the coating polymer (PDA), naturally enters living cells unaided by transfection agents, resists enzymatic lysis and shows high biocompatibility. This nanoplatform design contributes towards future biomolecule analytical method development based on polymer decorated/modified 2D nanomaterials.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fósforo
/
Polímeros
/
Espectrometría de Fluorescencia
/
Trombina
/
Calcio
/
Nanoestructuras
/
Indoles
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chim Acta
Año:
2020
Tipo del documento:
Article
País de afiliación:
China