Your browser doesn't support javascript.
loading
Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori).
Shi, Guiqin; Shen, Jiaxin; Ren, Fei; Yang, Weikai.
Afiliación
  • Shi G; College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, China.
  • Shen J; College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, China.
  • Ren F; College of Mechanical and Electrical Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.
  • Yang W; College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, China.
Arch Insect Biochem Physiol ; 106(1): e21744, 2021 Jan.
Article en En | MEDLINE | ID: mdl-32989839
Superoxide dismutases (SODs) play an essential role in eliminating excess reactive oxygen species and maintaining the redox balance of the immune system. To study the function of BmSOD3 in silkworm, 543-bp full-length complementary DNA-encoding BmSOD3 was cloned from silkworm. The BmSOD3 amino acids were compared to their homologs, and several highly conserved regions were analyzed. We also carried out phylogenetic analyses of the SOD gene. Our results showed that the BmSOD3 gene belonged with the ecCu/Zn SOD gene. The BmSOD3 gene was transformed into the pET28a vector for functional expression in Escherichia coli. The sodium salt-polyacrylamide gel electrophoresis results showed that the molecular weight of recombinant BmSOD3 was about 22 kDa. The recombinant protein BmSOD3 was purified to detect its properties. After purification analyses, the enzyme activity showed Cu/Zn SOD activity, and the specific activity of the purified enzyme was 0.51 U/mg. The BmSOD3 transcripts showed tissue-specific expression in the midgut and malpighian tubule. The immune microarray data for BmSOD3 showed an expression signal that had a strong response to the induction of four pathogens (Bacillus bombyseptieus, Beauveria bassiana, E. coli, and nuclear polyhedrosis virus), particularly after infection for 24 h, which indicates that the BmSOD3 gene plays a key role in response to bacterial, fungal, and viral invasion. The fusion protein also showed antibacterial activity against E. coli in vitro. Thus, the fusion protein BmSOD3 exhibits antibacterial activity and may be used in production to combat diseases caused by bacteria in silkworm.
Asunto(s)
Palabras clave

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bombyx / Superóxido Dismutasa Idioma: En Revista: Arch Insect Biochem Physiol Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bombyx / Superóxido Dismutasa Idioma: En Revista: Arch Insect Biochem Physiol Año: 2021 Tipo del documento: Article País de afiliación: China