Para rubber seed oil: The safe and efficient bio-material for hair loss treatment.

ABSTRACT

OBJECTIVES: Para rubber (Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.)) is the important crop of the word. It has been vastly used in biomedical products. However, its pharmacologically application besides the latex is sparely to be explored especially the seed. Cellular biological activities of the standardized para rubber seed oil for hair loss treatment were therefore assessed. METHODS: Para rubber seed oil was prepared and standardized using GC/MS on the basis of its pharmacologically active fatty acids. The oil was safety assessed in human dermal papilla and DU-145 human prostate carcinoma. Cellular antioxidant activity was determined as well as proliferation stimulating efficacy and inhibitory effect against 5α-reductase. RESULTS: Oleic acid, fatty acid of cutaneous benefits, was majorly detected in the oil and followed by linoleic, palmitic, and stearic acids. The standardized para rubber seed oil was proved to be safe on human follicle dermal papilla and DU-145 human prostate carcinoma at the concentration of 0.1-50 and 0.1-100 µg/mL, respectively. The standardized para rubber seed oil stimulated the cell proliferation and posed cellular antioxidant activity in human dermal papilla at a comparable potency to minoxidil, dutasteride and vitamin C at the same tested concentration. In addition, the standardized para rubber seed oil inhibited 5α-reductase as examined in DU-145 human prostate carcinoma, although at a lesser degree than the standards at the same tested concentration. CONCLUSIONS: The standardized para rubber seed oil is evidenced as the safe and efficient bio-oil to be used for hair growth stimulating or reduce/suppress hair loss treatment.