Effects of plants extracts on the expression of major genes of JAK/STAT pathway.
Nucleosides Nucleotides Nucleic Acids
; 40(4): 434-469, 2021.
Article
en En
| MEDLINE
| ID: mdl-33749513
This study analyzed the effects of the plant extracts (Citrus limon, Solanum lycopersicum, Zingiber officinale, Vitis vinifera and Allium sativum) on the growth of mammalian cells (Vero and MDA-MB-231) and evaluated the most effective plant extract for the expression of specific genes of the JAK/STAT pathway in human breast cancer cells. An antiproliferative bioassay involving neutral red-dye uptake was used to determine the anticancerous potential of plant extracts. In Vero cells, the ginger methanolic extract was least effective; whereas the lemon methanolic extract was more effective with 64 dilutions with IC50 51.42%. In MDA-MB-231 cells, the tomato and ginger methanolic, and grape water extracts were least effective, whereas lemon water extract was most effective with 32 dilutions with IC50 48.67%, by upregulating JAK1, JAK2, TYK2, IRF7 and IRF3 gene expressions of the JAK/STAT pathway. C. limon inhibited the growth of both Vero and MDA-MB 231 cells. It suggested that C. limon has anti-cancer potential by inducing the JAK/STAT pathway.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
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Citrus
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Factores de Transcripción STAT
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Quinasas Janus
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Antineoplásicos Fitogénicos
Idioma:
En
Revista:
Nucleosides Nucleotides Nucleic Acids
Año:
2021
Tipo del documento:
Article
País de afiliación:
Canadá