In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy.

Zuhayri, Hala; Nikolaev, Viktor V; Knyazkova, Anastasia I; Lepekhina, Tatiana B; Krivova, Natalya A; Tuchin, Valery V; Kistenev, Yury V

Zuhayri, Hala; Nikolaev, Viktor V; Knyazkova, Anastasia I; Lepekhina, Tatiana B; Krivova, Natalya A; Tuchin, Valery V; Kistenev, Yury V.

Afiliación

Zuhayri H; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Nikolaev VV; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Knyazkova AI; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Lepekhina TB; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Krivova NA; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Tuchin VV; Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia.
Kistenev YV; Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia.

Pharmaceutics ; 14(2)2022 Jan 26.

Article en En | MEDLINE | ID: mdl-35214020

ABSTRACT

ABSTRACT

The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm2). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60-80 µm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm2.

Palabras clave

low-dose photodynamic therapy; photosensitizer; second-harmonic-generation-to-auto-fluorescence aging index of the dermis; two-photon microscopy; wound healing

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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2022 Tipo del documento: Article País de afiliación: Rusia