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A Selenium-based Cysteine Surrogate for Protein Chemical Synthesis.
Firstova, Olga; Agouridas, Vangelis; Diemer, Vincent; Melnyk, Oleg.
Afiliación
  • Firstova O; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
  • Agouridas V; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France.
  • Diemer V; Centrale Lille; F-59000, Lille, France.
  • Melnyk O; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, France. vincent.diemer@ibl.cnrs.fr.
Methods Mol Biol ; 2530: 213-239, 2022.
Article en En | MEDLINE | ID: mdl-35761052
ABSTRACT
N-selenoethyl cysteine (SetCys) in the form of its cyclic selenosulfide is a cysteine surrogate, whose reactivity depends on the reducing power of the medium. SetCys does not interfere with the native chemical ligation reaction under mild reducing conditions, that is in the absence of tris(2-carboxyethyl)phosphine (TCEP). In contrast, subjecting SetCys to TCEP results in the spontaneous loss of its N-selenoethyl appendage and thus to its conversion into a Cys residue. Therefore, SetCys can be used for the redox-controlled assembly of peptide segments using NCL. We provide in this protocol detailed procedures for the synthesis of Fmoc-protected SetCys residue and for its incorporation into peptides using standard solid-phase peptide synthesis protocols. We also describe its use for the chemical synthesis of proteins through the redox-controlled assembly of three peptide segments in one-pot.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Selenio / Cisteína Idioma: En Revista: Methods Mol Biol Año: 2022 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Selenio / Cisteína Idioma: En Revista: Methods Mol Biol Año: 2022 Tipo del documento: Article País de afiliación: Francia