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Dephytinization of wheat and rice bran by cross-linked enzyme aggregates of Mucor indicus phytase: a viable prospect for food and feed industries.
Venkataraman, Swethaa; Vaidyanathan, Vinoth Kumar.
Afiliación
  • Venkataraman S; Integrated Bioprocessing Laboratory, Department of Biotechnology, SRM Institute of Science and Technology, Chennai, India.
  • Vaidyanathan VK; Integrated Bioprocessing Laboratory, Department of Biotechnology, SRM Institute of Science and Technology, Chennai, India.
J Sci Food Agric ; 103(4): 1935-1945, 2023 Mar 15.
Article en En | MEDLINE | ID: mdl-36408806
ABSTRACT

BACKGROUND:

Novel feeds for improved feed intake and for enhanced nutrient bioavailability have recently attracted attention. Insoluble dietary fibers, especially rice and wheat bran, have generated much interest due to their nutritional value. Incorporating insoluble dietary fiber into diets could be a viable way to maximize the feed conversion ratio.

RESULTS:

Cross-linked phytase aggregates (CLPA) were synthesized by precipitating enzymes followed by cross-linking with 5 mmol L-1 glutaraldehyde, yielding 88.24 (U g-1 ) of enzyme load without the assistance of a proteic feeder. The epitome of the study is the dephosphorylation of wheat bran and rice bran by varying pH, enzyme concentration, and temperature. The highest inorganic phosphorus liberation by 150 U L--1 of free phytase was 23.72 (wheat bran) and 48.08 mg g-1 (rice bran) after 12 h of incubation.  Furthermore, 150 U L-1 of CLPA liberated 28.72 (wheat bran) and 52.08 mg g-1 (rice bran) of inorganic phosphorus with an incubation time of 12 h.

CONCLUSION:

Thermostable free phytase was insolubilized to dephosphorylate the agro-residue, namely, wheat bran and rice bran, to reduce the anti-nutritional factor (the phytate content) of these insoluble dietary fibers. © 2022 Society of Chemical Industry.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Oryza / 6-Fitasa Idioma: En Revista: J Sci Food Agric Año: 2023 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Oryza / 6-Fitasa Idioma: En Revista: J Sci Food Agric Año: 2023 Tipo del documento: Article País de afiliación: India