Broad-specificity monoclonal antibody against neonicotinoid insecticides via a multi-immunogen strategy and development of a highly sensitive GNP-based multi-residue immunoassay in ginseng and tomato.
Food Chem
; 420: 136115, 2023 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-37062080
ABSTRACT
Neonicotinoid insecticides (NNIs) are extensively used across the agricultural products and foods. In order to meet the rapid detection requirements, a novel broad-specificity monoclonal antibody against NNIs was developed for the first time using a multi-immunogen strategy. The antibody's high affinity and its ability to bind target molecules were verified by ic-ELISA. Furthermore, molecular docking was used to evaluate the pivotal forces affecting binding affinity and to determine binding sites. Subsequently, a highly sensitive gold nanoparticle-based immunochromatographic assay was established for the rapid detection of eight NNIs and the IC50 values were 0.03-1.61 ng/mL. The limits of detection for ginseng and tomato ranged from 0.76 to 30.19 µg/kg and 0.87 to 31.57 µg/kg, respectively. The spiked recovery ranged from 72.04% to 120.74%, and the coefficient of variation were less than 9.0%. This study provides a new direction for the development of multiple NNIs residue immunoassays.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nanopartículas del Metal
/
Insecticidas
/
Anticuerpos Monoclonales
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Food Chem
Año:
2023
Tipo del documento:
Article
País de afiliación:
China