Glycyrrhizic acid treatment ameliorates anxiety-like behaviour via GLT1 and Per1/2-dependent pathways.
J Ethnopharmacol
; 328: 118013, 2024 Jun 28.
Article
en En
| MEDLINE
| ID: mdl-38453099
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE As a traditional Chinese medicinal herb, Glycyrrhiza. URALENSIS Fisch. (licorice root, chinese name Gancao) has a variety of medicinal values and is widely used clinically. Its main active ingredient, glycyrrhizic acid (GA), is believed to have a neuroprotective effect. However, the underlying biological mechanisms of GA on stress-induced anxiety disorders are still unclear. AIM OF THE STUDY To investigate the anti-anxiety effect of GA and its underlying mechanism. METHODS:
We selected the anxiety model induced by repeated chronic restraint stress (CRS) for 2 h on each of 7 consecutive days. GA (4, 20, 100 mg/kg) was injected intraperitoneally once daily for 1 week. The potential GA receptors were identified using whole-cell patches and computer-assisted docking of molecules. High-throughput RNA sequencing, adeno-associated virus-mediated gene regulation, Western blotting, and RT-qPCR were used to assess the underlying molecular pathways.RESULTS:
GA alleviate depression-like and anxiety-like behaviors in CRS mice. GA decreased synaptic transmission by facilitating glutamate reuptaking in mPFC. Meanwhile, long-term GA treatment increased the expression of clock genes Per1 and Per2. Suppressing both Per1 and Per2 abolished the anxiolytic effects of GA treatment.CONCLUSION:
Our study suggests that GA may be developed for the treatment of stress-induced anxiety disorders, and its mechanism is related to GLT1 and Per1/2-dependent pathways. This presents a novel approach to discovering potent therapeutic drugs.Palabras clave
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Bases de datos:
MEDLINE
Métodos Terapéuticos y Terapias MTCI:
Plantas_medicinales
Asunto principal:
Ácido Glicirrínico
/
Antioxidantes
Idioma:
En
Revista:
J Ethnopharmacol
Año:
2024
Tipo del documento:
Article