Methods of increasing the sensitivity of the haemagglutination inhibition test for rabies virus antibody.
Bull World Health Organ
; 62(6): 883-92, 1984.
Article
en En
| MEDLINE
| ID: mdl-6335846
ABSTRACT
A new method for the removal of non-specific inhibitors of rabies virus haemagglutinin has been developed. Treatment with colloidal silicic acid (Aerosil) or with acetone plus Aerosil reduced the non-specific inhibitors in human, mouse, and dog sera to a level that was undetectable at the 14 starting dilution in the haemagglutination inhibition test.Bromelain-treated goose erythrocytes were much more susceptible to haemagglutination by rabies virus than were untreated erythrocytes, and the sensitivity of the haemagglutination inhibition test was considerably increased by using bromelain-treated erythrocytes. Low levels of antibodies in sera from immunized human subjects were detected with higher sensitivity by combining Aerosil treatment of the sera with the use of bromelain-treated goose erythrocytes in the haemagglutination inhibition test.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Rabia
/
Virus de la Rabia
/
Pruebas de Inhibición de Hemaglutinación
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Bull World Health Organ
Año:
1984
Tipo del documento:
Article