Identification by mutational analysis of four critical residues in the molybdenum cofactor domain of eukaryotic nitrate reductase.
FEBS Lett
; 370(3): 197-202, 1995 Aug 21.
Article
en En
| MEDLINE
| ID: mdl-7656976
ABSTRACT
The nucleotide sequence of the nitrate reductase (NR) molybdenum cofactor (MoCo) domain was determined in four Nicotiana plumbaginifolia mutants affected in the NR apoenzyme gene. In each case, missense mutations were found in the MoCo domain which affected amino acids that were conserved not only among eukaryotic NRs but also in animal sulfite oxidase sequences. Moreover an abnormal NR molecular mass was observed in three mutants, suggesting that the integrity of the MoCo domain is essential for a proper assembly of holo-NR. These data allowed to pinpoint critical residues in the NR MoCo domain necessary for the enzyme activity but also important for its quaternary structure.
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Bases de datos:
MEDLINE
Asunto principal:
Plantas Tóxicas
/
Pteridinas
/
Nicotiana
/
Coenzimas
/
Metaloproteínas
/
Nitrato Reductasas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
FEBS Lett
Año:
1995
Tipo del documento:
Article
País de afiliación:
Francia