Molecular cloning of rat RAC protein kinase alpha and beta and their association with protein kinase C zeta.
Biochem Biophys Res Commun
; 205(1): 817-25, 1994 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-7999118
ABSTRACT
cDNAs of RAC protein kinases (RAC-PK) alpha and beta were cloned from a rat testis cDNA library. The predicted open reading frames encode 480 and 481 amino acids of RAC-PK alpha and beta, respectively, and the rat RAC-PK alpha and beta have sequences conserved among different mammalian species such as the pleckstrin homology domain at their amino-terminal region and the protein-serine/threonine kinase catalytic domain at their carboxyl-terminal region. RNA blot analysis showed wide distribution of two RAC-PK in rat tissues. Immunoprecipitation analysis revealed that RAC-PK alpha and beta associate with protein kinase C zeta through the pleckstrin homology domain in vitro, suggesting the interaction between RAC-PK and protein kinase C.
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Bases de datos:
MEDLINE
Asunto principal:
Proteína Quinasa C
/
Proteínas Serina-Treonina Quinasas
/
Isoenzimas
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1994
Tipo del documento:
Article
País de afiliación:
Japón