Zinc is required for the expression of ornithine decarboxylase in a difluoromethylornithine-resistant cell line.
Biochem J
; 299 ( Pt 2): 515-9, 1994 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-8172613
ABSTRACT
Dilution of quiescent L1210-DFMOr (difluoromethylornithine-resistant) cells in fresh medium containing serum led to the induction of ornithine decarboxylase (ODC) and to the expression of its mRNA, as determined by a sensitive solution-hybridization-RNase-protection assay. Addition of the chelating agent diethylenetriaminepentaacetic acid (DTPA) at seeding time caused an inhibition of the induction of ODC activity by up to 90%, and only Zn2+ of the bivalent metal ions tested was effective in reversing this effect. The inhibition of the induction of ODC activity was accompanied by a marked decrease, prevented by Zn2+ supplementation, of the accumulation of immunoreactive ODC protein and ODC mRNA. DTPA treatment also caused a slight acceleration of ODC turnover. These results indicate that a restricted Zn2+ availability in L1210-DFMOr cells impairs ODC induction remarkably, mainly by affecting the expression of the messenger.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ornitina Descarboxilasa
/
Zinc
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Resistencia a Medicamentos
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Leucemia L1210
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Expresión Génica
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Eflornitina
Idioma:
En
Revista:
Biochem J
Año:
1994
Tipo del documento:
Article
País de afiliación:
Italia