Targeting aequorin and green fluorescent protein to intracellular organelles.
Gene
; 173(1 Spec No): 113-7, 1996.
Article
en En
| MEDLINE
| ID: mdl-8707049
ABSTRACT
Two proteins of Aequorea victoria were molecularly engineered and produced in mammalian cells, in order to serve as specific reporters of subcellular microenvironments. Aequorin (AEQ), a Ca(2+)-sensitive photoprotein, was successfully targeted to three intracellular locations cytosol, nucleus and mitochondria. The recombinant apoprotein, reconstituted into active AEQ by the addition of the prosthetic group to the culture medium, allows the direct measurement of [Ca2+] within those compartments, thus directly addressing questions of large biological interest. The same approach was utilized for the green fluorescent protein (GFP) for specific labelling, in vivo, of the various subcellular structures. GFP was targeted to mitochondria the recombinant protein, strongly fluorescent in a highly reducing environment, provides a powerful tool for visualizing these organelles in living cells, and may represent the prototype of a new family of intracellularly targeted fluorescent probes.
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Bases de datos:
MEDLINE
Asunto principal:
Orgánulos
/
Aequorina
/
Proteínas Luminiscentes
Idioma:
En
Revista:
Gene
Año:
1996
Tipo del documento:
Article
País de afiliación:
Italia