The effect of external Ca2+ and Ca(2+)-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L.
Cell Res
; 8(1): 41-50, 1998 Mar.
Article
en En
| MEDLINE
| ID: mdl-9570016
ABSTRACT
Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean (Phaseolus radiatus L.) was observed only when Ca2+ ions were present in the medium. The optimal CaCl2 concentration was 250 microM. Swelling response declined when Ca2+ was supplied into the medium after red light irradiation. The Ca(2+)-chelator EGTA eliminated the red-light-induced swelling and 45Ca2+ accumulation in the protoplasts. In contrast, A23187, a Ca(2+)-ionophore, could mimic the effect of red light in darkness. These results indicate that Ca2+ may play a role in light signal transduction. In addition, swelling response was prevented by TFP and CPZ (both are CaM antagonists), implying the involvement of CaM in red-light-induced and Ca(2+)-dependent protoplast swelling.
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Bases de datos:
MEDLINE
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Plantas Medicinales
/
Protoplastos
/
Canales de Calcio
/
Calcio
/
Fabaceae
/
Luz
Idioma:
En
Revista:
Cell Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
China