Human IgE production in hu-PBL-SCID mice injected with birch pollen and diesel exhaust particles.
Toxicology
; 128(3): 219-30, 1998 Jul 17.
Article
en En
| MEDLINE
| ID: mdl-9750044
ABSTRACT
Mice with severe combined immunodeficiency were transplanted with human peripheral blood lymphocytes (hu-PBL-SCID mice). The response to immunisation with birch pollen was used to study possible effects of diesel exhaust particles (DEP) and aluminium hydroxide (Al(OH)3) on human IgE production in this human in vivo model. The adjuvants were well tolerated, as determined by the number of human cells in the peritoneal cavity at the end of the experiments. Total and birch pollen-specific IgE was detected in 76 and 41% of the mice, respectively. In the present experiments where the mice were stimulated early with birch pollen, a doubling in percentage of hu-PBL-SCID mice with production of specific IgE was observed, as compared to later stimulation used in previous experiments. Although a tendency to higher total IgE levels was observed after treatment with DEP, no statistically significant adjuvant effect of DEP or Al(OH)3 could be demonstrated. Electron microscopy analysis after immunogold labelling showed that the major birch pollen allergen Bet v I was released from the pollen grains and adsorbed to the surface of the DEP. Early stimulation with allergen appears to be important for optimal production of specific IgE in the hu-PBL-SCID model. However, our results show that further improvements are needed in order to demonstrate the expected effects from adjuvants and environmental pollutants.
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Bases de datos:
MEDLINE
Asunto principal:
Polen
/
Árboles
/
Emisiones de Vehículos
/
Inmunoglobulina E
/
Alérgenos
Idioma:
En
Revista:
Toxicology
Año:
1998
Tipo del documento:
Article
País de afiliación:
Noruega