Evaluation of genetic diversity of Dendrobium officinale wild populations based on RAMP markers / 药学学报
Acta Pharmaceutica Sinica
; (12): 1156-1160, 2011.
Article
en Zh
| WPRIM
| ID: wpr-233019
Biblioteca responsable:
WPRO
ABSTRACT
Random amplified microsatellite polymorphism (RAMP) markers were used to access the genetic diversity among 112 samples of nine populations of Dendrobium officinale Kimura et Migo. Using 16 informative primers, 123 bands were amplified and 86 (69.92%) were polymorphic. The polymorphic bands from three to eight could be detected for each RAMP primer, with a mean of 5, indicating abundant genetic diversity among populations. Genetic similarity coefficients ranged from 0.250 to 0.813. UPGMA dendrogram illustrated 9 populations clustered into 3 groups, and the cluster pattern showed correlation with the locations of the D. officinale populations. These results were supported by the previous conclusions that were achieved by other molecular markers, and RAMP is proved to be effective for evaluating the genetic diversity of wild populations of Dendrobium officinale.
Texto completo:
1
Bases de datos:
WPRIM
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Filogenia
/
Plantas Medicinales
/
Polimorfismo Genético
/
Variación Genética
/
Análisis por Conglomerados
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
ADN de Plantas
/
Técnica del ADN Polimorfo Amplificado Aleatorio
/
Repeticiones de Microsatélite
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2011
Tipo del documento:
Article