Effect of glycogen on calcium of donor liver during ischemia-reperfusion period / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 21-24, 2002.
Article
en Zh
| WPRIM
| ID: wpr-257898
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.</p><p><b>METHODS</b>Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.</p><p><b>RESULTS</b>Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.</p><p><b>CONCLUSIONS</b>Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.</p>
Texto completo:
1
Bases de datos:
WPRIM
Asunto principal:
Fisiología
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Daño por Reperfusión
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Adenosina Trifosfato
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Química
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Calcio
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Trasplante de Hígado
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ATPasas Transportadoras de Calcio
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Modelos Animales
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Citosol
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Glucógeno
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2002
Tipo del documento:
Article