Experimental study on effect of baoganning on activity of nuclear transcription factor-kappaB in hepatic stellate cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 804-807, 2005.
Article
en Zh
| WPRIM
| ID: wpr-269894
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Baoganning (BGN) on activity of nuclear transcription factor-kappaB (NF-kappaB) in hepatic stellate cells (HSCs) and its relevant mechanisms.</p><p><b>METHODS</b>Normal Wistar rats were medicated with BGN decoction by gavage for 7 days to prepare BGN drug-serum. The effect of BGN drug-serum on HSC-T6 growth was measured by MTT assay; phosphorylation level of NF-kappaB inhibiting factor IkappaB at different time after BGN stimulation was detected by Western blotting analysis; and the binding level of NF-kappaB with DNA was measured 30 min after drug-serum stimulation with gel shift assay.</p><p><b>RESULTS</b>BGN could significantly inhibit the HSC-T6 growth and quickly supress the phosphorylation of IkappaB, with the effect reached its peak at 30 min and restored to baseline level 6 h after stimulation, and reduce the binding capacity of NF-kappaB with DNA.</p><p><b>CONCLUSION</b>BGN can inhibit phosphorylation of IkappaB, restrain the activity of NF-kappaB and change the binding level of NF-kappaB with DNA.</p>
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Farmacología
/
Fosforilación
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ADN
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Medicamentos Herbarios Chinos
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Distribución Aleatoria
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Células Cultivadas
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FN-kappa B
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Ratas Wistar
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Hepatocitos
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Biología Celular
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2005
Tipo del documento:
Article