Hydroxysafflor yellow A up-regulates HIF-1alpha via inhibition of VHL and p53 in Eahy 926 cell line exposed to hypoxia / 药学学报
Acta Pharmaceutica Sinica
; (12): 484-489, 2008.
Article
en Zh
| WPRIM
| ID: wpr-277826
Biblioteca responsable:
WPRO
ABSTRACT
In present study, we investigated the mechanism of regulating HIF-1alpha expression by hydroxysafflor yellow A (HSYA) in Eahy 926 cell line under 1% O2 hypoxia. Eahy 926 cells were incubated with HSYA (100, 10 and 1 micromol x L(-1)) under hypoxia for the indicated time after treatment. Cell proliferation rate was detected using MTT assays. VHL and p53 location and protein expression were analyzed by immunocytochemical stain. HIF-1alpha, VHL and p53 mRNA expression were detected by RT-PCR. Protein expression of HIF-1alpha, VHL and p53 were assayed by Western blotting method. HSYA at 100 micromol x L(-1) increased Eahy 926 cells proliferation rate under hypoxia. HIF-1alpha mRNA and protein expression were up-regulated in the presence of HSYA. VHL, p53 mRNA and protein expression decreased significantly after 8 hours of treatment under hypoxia. HSYA protected Eahy 926 cells from hypoxia, and up-regulated HIF-1alpha expression partially via its inhibition of VHL and p53 expression.
Texto completo:
1
Bases de datos:
WPRIM
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Farmacología
/
Plantas Medicinales
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Quinonas
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Venas Umbilicales
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ARN Mensajero
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Hipoxia de la Célula
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Regulación hacia Arriba
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Línea Celular
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Chalcona
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Química
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2008
Tipo del documento:
Article