Study on HPLC fingerprint of Prunella vulgaris / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.</p><p><b>METHOD</b>A sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.</p><p><b>RESULT</b>The similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.</p><p><b>CONCLUSION</b>This method is available for quality evaluation and control the quality of P. vulgaris.</p>