Authentication of Pinellia pedatisecta and their adulterants by AS-PCR / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1109-1111, 2008.
Article
en Zh
| WPRIM
| ID: wpr-295402
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a molecular method for the authentication of Pinellia pedatisecta and its adulterants.</p><p><b>METHOD</b>DNA sequences of some species from P. tenore, Typhonium and Arisaema were downloaded from GenBank, the sequences were aligned using DNAMAN. Allele-specific primers for P. pedatisecta and P. tenore were designed according to their SNPs in rpl 20 sequence. The designed primers were used to amplify 10 samples of P. pedatisecta, P. ternata and T. flagelliforme.</p><p><b>RESULT</b>A 351 bp band was amplified from P. pedatisecta but not form P. ternata and T. flagelliforme by primer Pprpl149F and Pprpl484R. A 630 bp band was amplified from P. ternate and P. pedatisecta but not from T. flagelliforme by primer Ptrpl94F and Ptrpl699R.</p><p><b>CONCLUSION</b>AS-PCR has the advantages of highly specific and good reproducibility, by which P. pedatisecta can be identified from part of its adulterants quickly. It is a potential method to be used in the molecular identification of other materia medica.</p>
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Complementárias:
Homeopatia
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Plantas Medicinales
/
Control de Calidad
/
China
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Seguridad de Productos para el Consumidor
/
Polimorfismo de Nucleótido Simple
/
Pinellia
/
Alelos
/
Genética
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2008
Tipo del documento:
Article