A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1173-1179, 2009.
Article
en Zh
| WPRIM
| ID: wpr-296941
Biblioteca responsable:
WPRO
ABSTRACT
Quorum sensing is an important gene regulatory mechanism in Pseudomonas aeruginosa and controls the expression of numerous virulence factors. We designed and constructed a screening system for quorum-sensing inhibitors. We developed the system by using the lasI and rhlA promoters fused with promoterless sacB as reporters. Using this system we screened a number of Chinese herb extracts, and identified three herb extracts containing inhibitors to the quorum-sensing system and to its regulated genes. The screening system developed was highly efficient and sensitive. It could serve as a useful tool to identify herb compounds that block infections but unlikely render antibiotic resistance in pathogens.
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Farmacología
/
Pseudomonas aeruginosa
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Medicamentos Herbarios Chinos
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Química
/
Regiones Promotoras Genéticas
/
Genes Reporteros
/
Acanthaceae
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Valerianaceae
/
Evaluación Preclínica de Medicamentos
/
Percepción de Quorum
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Article