Simultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detection / 药学学报
Acta Pharmaceutica Sinica
; (12): 56-59, 2004.
Article
en En
| WPRIM
| ID: wpr-301149
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine A, imperialine-3 beta-glucoside and yibeinoside A.</p><p><b>METHODS</b>A Nova-Pak C18 column (150 mm x 3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL.min-1.</p><p><b>RESULTS</b>The linear range of peimissine was 13.1-288.2 mg.L-1 (r2 = 0.9975), imperialine-3 beta-glucoside 7.7-169.4 mg.L-1 (r2 = 0.9993), yibeinoside A 7.3-160.6 mg.L-1 (r2 = 0.9997), imperialine 16.5-363.0 mg.L-1 (r2 = 0.9992), sinpeinine A 8.7-191.4 mg.L-1 (r2 = 0.9942).</p><p><b>CONCLUSION</b>The method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.</p>
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Plantas Medicinales
/
Medicamentos Herbarios Chinos
/
Cevanas
/
Química
/
Cromatografía Líquida de Alta Presión
/
Fritillaria
/
Alcaloides
/
Glucósidos
/
Métodos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Acta Pharmaceutica Sinica
Año:
2004
Tipo del documento:
Article