Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2531-2534, 2007.
Article
en Zh
| WPRIM
| ID: wpr-324333
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules.</p><p><b>METHOD</b>Absorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0.5% acetic acid water solution as mobile phase, ODS C(18) chromatography column (4.6 mm x 150 mm, 5 microm) as stationary phase, puerarin was detected at 248 nm.</p><p><b>RESULT</b>There is a good linear relationship for puerarin in the range 0.025-3.2 microg x mL-1, RSD < 10%, and the average recovery is 97.6%.</p><p><b>CONCLUSION</b>The method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.</p>
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Asunto principal:
Farmacología
/
Plantas Medicinales
/
Saponinas
/
Sangre
/
Aceites Volátiles
/
Medicamentos Herbarios Chinos
/
Farmacocinética
/
Distribución Aleatoria
/
Isquemia Encefálica
/
Química
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Article