Identification of metabolites of antitumor lead compound T-OA in rat urine by HPLC-HRMS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 911-915, 2014.
Article
en Zh
| WPRIM
| ID: wpr-330337
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the major metabolites of antitumor lead compound T-OA (oleanolic acyl-3, 5, 6-trimethyl pyrazine-2-methyl ester) in rat urine, in order to preliminarily infer its metabolic mode in rats.</p><p><b>METHOD</b>Rat urines of the blank group, the raw material group (ligustrazine TMP and oleanolic acid OA Moore equivalent) and the T-OA group were collected and freeze-dried; Solids were extracted by ethyl acetate; And then the extracts were re-dissolved with acetonitrile. HPLC-HRMS coupling technique was adopted to find the potential mass spectrum peak under ESI(+) (see symbol) ESI(-) modes. Metabolite-related information was obtained by comparing the three groups of spectra.</p><p><b>RESULT</b>One metabolite of OA and two metabolites of TMP were identified in the raw material group; none metabolite of T-OA but one phase II metabolite was detected in the T-OA group.</p><p><b>CONCLUSION</b>It is the first time to identify one phase II metabolite of T-OA and one phase II metabolite of OA were identified in rat urine. On that basis, the researchers preliminarily inferred that T-OA does not show the efficacy in the form of raw material. The HPLC-HRMS method established could be used to identify metabolites of related derivative structures. This paper could also provide certain reference for designing pro-drugs based on oleanolic acid.</p>
Texto completo:
1
Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Espectrometría de Masas
/
Orina
/
Medicamentos Herbarios Chinos
/
Estructura Molecular
/
Química
/
Cromatografía Líquida de Alta Presión
/
Ratas Sprague-Dawley
/
Metabolismo
/
Métodos
/
Antineoplásicos
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Article