Rapid identification of medicinal herbs through plant Direct-PCR / 药学学报
Acta Pharmaceutica Sinica
; (12): 1763-1769, 2017.
Article
en Zh
| WPRIM
| ID: wpr-779787
Biblioteca responsable:
WPRO
ABSTRACT
Direct-PCR technology was using a 15 minutes heat-lysis step instead of DNA extraction to get DNA templates with small amount of plant materials followed by sensitive PCR process to amplify target genes. In order to facilitate DNA barcoding in medicinal herb identification with Direct-PCR, we collected different tissues from 80 medicinal plants as material to amplify the ITS fragments. Through optimizing the PCR reaction, ITS of 80 plant samples was all successfully amplified. PCR products were sequenced and to do Blast analysis. These results suggest that Direct-PCR would improve the efficiency of DNA barcoding in the application of medicinal herb molecular authentication.
Texto completo:
1
Bases de datos:
WPRIM
Métodos Terapéuticos y Terapias MTCI:
Terapias_biologicas
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2017
Tipo del documento:
Article