Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 519-524, 2013.
Article
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| WPRIM
| ID: wpr-812327
Biblioteca responsable:
WPRO
ABSTRACT
AIMS@#To investigate the antitumor effects of extracts from Oxytropis falcata on human hepatocellular carcinoma SMMC-7721 cells in vitro and in transplanted murine H22 tumors in vivo.@*METHODS@#Cell proliferation, cell cycle distribution and apoptosis in SMMC-7721 cells were determined and tumor growth inhibition in H22 tumors was investigated. Cell cycle distribution was analyzed by flow cytometry with propidium iodide (PI) and Annexin V-FITC/ PI double staining.@*RESULTS@#MTT assay revealed that essential oil and flavonoids of O. falcata (named EOOF and FOF) inhibited proliferation of SMMC-7721 cells in a dose-dependent manner. The IC50 value of EOOF and FOF were 0.115 and 0.097 mg·mL(-1), respectively. Cell cycle was arrested at G(1) phase, and induction of apoptosis occurred in SMMC-7721 cells when subjected to FOF. Growth inhibition in H22 solid tumors transplanted mice was significantly pronounced after being treated with FOF, and the inhibition ratio were 56.1% and 70.8% at the concentration of 30 and 60 mg·kg(-1).@*CONCLUSION@#The results suggest that FOF promotes apoptosis in SMMC-7721 cells and inhibits H22 tumor growth, resulting in a potential antitumor effect on hepatic cancer.
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Bases de datos:
WPRIM
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
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Medicina_china
Asunto principal:
Medicamentos Herbarios Chinos
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Ciclo Celular
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Química
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Apoptosis
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Carcinoma Hepatocelular
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Oxytropis
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Línea Celular Tumoral
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Proliferación Celular
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Quimioterapia
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Inhibidores de Crecimiento
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2013
Tipo del documento:
Article