Adaptive evolution analysis of squalene synthase in medicinal plants of Araliaceae / 中草药

ABSTRACT

Objective: To analyze the adaptive evolution of squalene synthase (SS) in the medicinal plants of Araliaceae. Methods: The adaptive evolutionary analysis of 23 SS genes of seven medicinal plants of Araliaceae was carried out by using the branch model, site model, branch-site model, MEC model, SLAC, FEL, and REL of PAML software. Results: In the analysis of PAML and MEC models, most of the branches and loci were found to be under strong negative selection, and no positive selection sites were found. The analysis by SLAC, FEL, and REL also showed that there were a large number of negative selection sites, only 412P, 413N, and 415K were positive selection sites. Conclusion: This indicates that negative selection plays a leading role in SS gene of Araliaceae. The 412P, 413N, and 415K sites found may be involved in the activity of SS.