Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study
Acta cir. bras
; Acta cir. bras;35(3): e202000303, 2020. tab, graf
Article
em En
| LILACS
| ID: biblio-1130621
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon.Methods:
Thirty rats were randomly allocated to six groups (n=5 each) LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis.Results:
The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen.Conclusion:
Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.Palavras-chave
Texto completo:
1
Base de dados:
LILACS
Métodos Terapêuticos e Terapias MTCI:
Terapias_energeticas
Assunto principal:
Tendão do Calcâneo
/
Carotenoides
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Terapia com Luz de Baixa Intensidade
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Tendinopatia
/
Tenotomia
/
Hidroxibutiratos
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Acta cir. bras
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil